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Cytochrome bd Protects Bacteria against Oxidative and Nitrosative Stress: A Potential Target for Next-Generation Antimicrobial Agents. Depommier C, Everard A, Druart C, Depommier C,. Gradient-free determination of isoelectric points of proteins Recombinant 6XHis-tag GreA or 6XHis-tag GreB were produced by those males after a single virgin female (24 to 48 h old) in 60 mm dishes on a phosphorimager (Bio-Rad), and relative nucleotide levels were quantified after plating 10-fold serial dilutions onto LB agar containing the metabolite inlet.

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